Description
The innovative EASYspin plus system is ideal or rapid purification of genomic DNA-free total RNA from amylose/ polyphenol plants. The unique genomic DNA elimination columns effectively remove genomic DNA from RNA samples. No DNase digestion step is required. The purified total RNA can be directly used for reverse transcription and RT-PCR. The whole process is phenol/chloroform-free. The unique lysis buffer immediately lyses biological samples and inactivates RNase and DNase, Centrifugal precipitation to remove polysaccharides, polyphenols and secondary metabolites, and then lyse the mixture with ethanol to adjust RNA binding and adsorption to the genomic DNA removal column, then the RNA is selectively eluted and filtered, and the residual DNA adsorbed on the genomic DNA removal column cannot be eluted Throw away together with the column to remove DNA. Ethanol is added to the lysate to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica-membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica membrane with RNase-free H2O